dok  (CancerTools Org)

Journal: Brain Communications

Article Title: Dose escalation pre-clinical trial of novel DOK7-AAV in mouse model of DOK7 congenital myasthenia

doi: 10.1093/braincomms/fcaf046

Figure Lengend Snippet: DOK7 is expressed in diaphragm, triceps brachii and TA in AMP-101-treated Dok-7 KI/KI model mice. ( A ) Examples of western blots showing DOK7 expression. Robust expression is seen in muscles from many of the treated mice. Endogenous levels of DOK7 in WT mice are very low. ( B ) Densitometry was carried out to semi-quantify DOK7 expression. First, the ratio of the band intensity of DOK7 with total protein was calculated, and then this was normalized with a positive control (lysate from HEK292T cells transfected with human DOK7 cDNA). Each point represents a mouse, n = 4–5. P -values were obtained using a one-way ANOVA with Tukey’s multiple comparison test. Bars show mean values.

Article Snippet: Nitrocellulose was incubated in blocking buffer (PBS containing 3% powdered skimmed milk) for 30 min at RT and then incubated with primary mouse anti-DOK7 antibody (Santa Cruz, cat. no. sc-390856) diluted 1:250 in blocking buffer for 1 h at RT with gentle agitation.

Techniques: Western Blot, Expressing, Muscles, Positive Control, Transfection, Comparison